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| Chen,Feng; Chen,Xiang-Ming; Chen,Zhi; Jiang,Han-Liang; Pan,Xiao-Ping; Hu,Zhong-Rong; Liu,Rong-Hua; Chen,Xiao-Ming. |
| We want to construct a yeast expression system for thymosin a1 (Ta1) to make the orally administered Ta1 preparation possible. The whole Ta1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Ta1 in recombinant coincided with the original one reported in Genbank. When pYES2-Ta1 plasmid was transformed into yeast, galactose instead of glucose was used to induce Ta1 expression. Western blot was performed to identify the quality of the expressed Ta1. Dried yeast containing pYEST2-Ta1 was fed to Balb/c mice whose immunities were inhibited by cyclophosphamide in advance. Synthesized Ta1 peptide was used as positive control and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thymosin a1; Yeast expression system; Biological activity; Orally administered. |
| Ano: 2005 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300002 |
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