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| Lasa, Aide; Cesare, Andrea; Tassistro, Giovanni; Borello, Alessio; Gualdi, Stefano; Furones, Dolors; Carrasco, Noelia; Cheslett, Deborah; Brechon, Amanda; Paillard, Christine; Bidault, Adeline; Pernet, Fabrice; Canesi, Laura; Edomi, Paolo; Pallavicini, Alberto; Pruzzo, Carla; Vezzulli, Luigi. |
| Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 (OsHV‐1) have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve disease. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61952/66036.pdf |
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| Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.. |
| Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed.... |
| Tipo: Text |
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf |
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