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Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae) 74
Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F..
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005
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Temperature affects the hypoxia tolerance of neotropical Cichlid Geophagus brasiliensis 83
Pereira,Jéssica A.; Veronez,Alexandra C.S.; Coppo,Gabriel C.; Duca,Charles; Chippari-Gomes,Adriana R.; Gomes,Levy C..
ABSTRACT Oxygen and temperature are the most limiting factors in aquatic environments. Several species are exposed to variations of these factors in water because of physical, chemical and biological processes. The objective of this study was to evaluate the metabolic profile and the tolerance to the hypoxia of Geophagus brasiliensis exposed to changes in temperature and oxygen availability. The fish were exposed to 20 and 90% of oxygen saturation combined with different temperatures (20°, 24° and 28° C) for 8 h. Hepatic and muscular glycogen, as well as the activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), citrate synthase (CS) and their ratios were evaluated. Both glycogen and MDH activity showed a significant difference in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aerobic and anaerobic pathways; Behavior; Metabolism; Oxygen.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100202
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Acute copper toxicity in juvenile fat snook Centropomus parallelus (Teleostei: Centropomidae) in sea water 83
Oliveira,Bruno L.; Fernandes,Luiz F. Loureiro; Bianchini,Adalto; Chippari-Gomes,Adriana R.; Silva,Bruno F.; Brandão,Geisamanda P.; Gomes,Levy C..
Three experiments were designed to assess the accumulation and acute toxicity of copper (Cu) in juvenile fat snook Centropomusparallelus. The first experiment was performed to determine the 96-h lethal concentration (LC50) of Cu. The second experiment was designed to assess the effects of sublethal concentrations of Cu (0.47 and 0.94 mg/L), while the third one allowed us to test the recovery capacity of fish exposed to the sublethal concentrations Cu and kept in sea water without Cu addition. The LC50value for Cu was found to be 1.88 mg/L Cu. Fish exposed to the sublethal concentrations of Cu showed a significant accumulation of Cu in gills at 96 h respect to the control ones (0.43 µg/g Cu). No significant difference was observed in the accumulation of Cu...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic toxicology; Comet assay; Gills; Lethal toxicity; Micronucleus test.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400845
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