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	Provedor de dados ArchiMer (2) Autor Choi, Kwang-sik (2) Berthe, Franck (1) Jeung, Hee-do (1) Kang, Do-hyung (1) Le Moullac, Gilles (1) Ngo, Thao (1) Park, Heung-sik (1) Mais... Palavra-chave Black-lip pearl oyster (1) Crassostrea gigas (1) ELISA (1) Gonad development (1) Korea (1) Marteilioides chungmuensis (1) Ovary parasite (1) Pinctada margaritifera (1) Polyclonal antibody (1) Quantification of eggs and sperm (1) Mais... Tipo do documento Text (2) Ano 2014 (1) 2003 (1) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) ArchiMer Jeung, Hee-do; Kang, Do-hyung; Park, Heung-sik; Le Moullac, Gilles; Choi, Kwang-sik. We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to... Tipo: Text Palavras-chave: Black-lip pearl oyster; ELISA; Pinctada margaritifera; Polyclonal antibody; Quantification of eggs and sperm. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28659/32145.pdf Prevalence and infection intensity of the ovarian parasite Marteilioides chungmuensis during an annual reproductive cycle of the oyster Crassostrea gigas ArchiMer Ngo, Thao; Berthe, Franck; Choi, Kwang-sik. The occurrence of Marteilioides chungmuensis, a protozoan paramyxean parasite in the reproductive system of the Pacific oyster Crassostrea gigas, was observed at Gosung Bay, Korea. Seasonal variation in gonad development was investigated in a suspended cultured oyster population. Gametogenesis began in February and first-spawning was observed between mid and late June when surface water temperature reached 22 to 25degreesC. Spawning activity extended from mid June to late September, with 2 marked spawning peaks in June and August. Histological examination indicated that gonad development paralleled seasonal fluctuations in water temperature. Spawning in late June was partly associated with a sudden drop in salinity due to large freshwater inputs to the Bay... Tipo: Text Palavras-chave: Korea; Ovary parasite; Marteilioides chungmuensis; Gonad development; Crassostrea gigas. Ano: 2003 URL: https://archimer.ifremer.fr/doc/2003/publication-2848.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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