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Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration BJID
Chudobova,Dagmar; Cihalova,Kristyna; Guran,Roman; Dostalova,Simona; Smerkova,Kristyna; Vesely,Radek; Gumulec,Jaromir; Masarik,Michal; Heger,Zbynek; Adam,Vojtech; Kizek,Rene.
ABSTRACT BACKGROUND: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n = 50) presenting with bacterial infection. METHODS: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600604
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