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	Provedor de dados Nature Precedings (5) Autor Alessandra Pani (5) Claudia Norfo (5) Claudia Abete (4) Paolo La Colla (4) Claudia Mulas (3) Marirosa Putzolu (3) Sandra Dessì (3) Alessandra Mocali (2) Antonella Mandas (2) Fabrizio Angius (2) Francesco Paoletti (2) Giacomo Diaz (2) Sandra Dessi (2) Sergio Laconi (2) Anna Borgia (1) Christina Doriana Orrù (1) Christina Doriana Orru (1) Christina Zaru (1) Cristina D. Orrù (1) Enrico Peiretti (1) Mais... Palavra-chave Molecular Cell Biology (4) Neuroscience (3) Microbiology (1) Pharmacology (1) Tipo do documento Manuscript (5) Ano 2009 (2) 2008 (1) 2007 (2) País United Kingdom (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última ACAT1, Cav-1, and PrP expression in brains and skin fibroblasts from Sarda breed sheep with scrapie-resistant and scrapie-susceptible genotype. Nature Precedings Alessandra Pani; Marirosa Putzolu; Claudia Mulas; Cristina D. Orrù; Claudia Abete; Claudia Norfo; M. Dolores Cannas; Sergio Laconi; Paolo La Colla; Sandra Dessì. Scrapie is an infective ovine neurodegenerative disease; the only identified component of the infectious agent being an aberrant isoform (PrPSc) of the cellular prion protein (PrPC). So far, no means for ante-mortem diagnosis are available for Scrapie as well as for any other mammal Transmissible Spongiform Encephalopaties. We recently found a strong relationship between cell susceptibility to scrapie-infection and intracellular cholesterol homeostasis alterations. In brain tissues as well as in ex vivo cultures of skin fibroblasts and PBMCs from healthy and scrapie-affected sheep carrying a scrapie-susceptible (ARQ/ARQ) genotype, the levels of cholesterol esters were consistently higher than in tissues and cultures derived from animals with a... Tipo: Manuscript Palavras-chave: Molecular Cell Biology. Ano: 2007 URL: http://precedings.nature.com/documents/1211/version/1 Glucose-6-phosphate-dehydrogenase deficiency as a risk factor in proliferative disorder development Nature Precedings Antonella Mandas; Enrico Peiretti; Pierluigi Cocco; Claudia Norfo; Claudia Abete; Fabrizio Angius; Alessandra Pani; Giacomo Diaz; Sandra Dessì; Maurizio Fossarello. Glucose-6-phosphate dehydrogenase (G6PD) is an important site of metabolic control in the pentose phosphate pathway (PPP) which provides reducing power (NADPH) and pentose phosphates. The former is mainly involved in the detoxification of chemical reactive species; the latter in the regulation of cell proliferation. G6PD deficiency is the most common enzymopathy in the human population, characterized by decreased G6PD activity, mainly in red blood cells, but actually also in nucleated cells. This decreased activity is not due to enzyme synthesis impairment, but rather to reduced enzyme stability, which leads to a shortening of its half-life. Therefore, a major problem is to understand the underlying mechanisms linking G6PD deficiency to oxidative stress... Tipo: Manuscript Palavras-chave: Molecular Cell Biology. Ano: 2009 URL: http://precedings.nature.com/documents/3001/version/1 In vitro synergistic anti-prion effect of cholesterol ester modulators Nature Precedings Christina Doriana Orrù; Maria Dolores Cannas; Sarah Vascellari; Fabrizio Angius; Pier Luigi Cocco; Claudia Norfo; Antonella Mandas; Paolo La Colla; Sandra Dessì; Alessandra Pani. Background. Our studies on the role of cholesterol in prion infection/replication showed that brains and peripheral cells of sheep susceptible to or suffering from Scrapie were characterized by an altered cholesterol homeostasis compared to animals with a scrapie-resistant genotype, and that drugs influencing cholesterol esterification were endowed with selective anti-prion activity in N2a cell lines infected with the 22L and RML prion strains. 
Results. In prion-infected N2a cell lines we now report increased anti-prion activity of dual-drug combinations consisting of cholesterol ester modulators associated with prion inhibitors Synergism was obtained with the cholesterol ester modulators everolimus, pioglitazone, progesterone, and... Tipo: Manuscript Palavras-chave: Microbiology; Neuroscience; Pharmacology. Ano: 2009 URL: http://precedings.nature.com/documents/3883/version/1 Altered cholesterol ester cycle in _ex vivo_ skin fibroblasts from Alzheimer patients Nature Precedings Alessandra Pani; Sandra Dessi; Giacomo Diaz; Claudia Abete; Claudia Mulas; Claudia Norfo; Marirosa Putzolu; Maria Dolores Cannas; Christina Doriana Orru; Alessandra Mocali; Pier Luigi Cocco; Paolo La Colla; Francesco Paoletti. Recent studies in both animal and cell models of Alzheimer's disease (AD) indicated that sub-cellular cholesterol distribution seems to regulate amyloid-beta (A[beta]) generation in the brain. In particular, cholesterol-esters (CE), rather than total cholesterol levels, appear directly correlated with A[beta] production. Here we observed that, similarly to brain cells, skin fibroblasts obtained from AD patients produce and accumulate more CE than skin fibroblasts from age-matched healthy controls do. AD fibroblasts also exhibited a 2 fold increase in the expression of ACAT1, in addition to lower levels of SREBP2, nCEH, Caveolin-1 and ABCA1 mRNA levels, all of which are involved in the CE cycle. HMGCoA-reductase and LDL-receptor mRNAs levels did... Tipo: Manuscript Palavras-chave: Molecular Cell Biology; Neuroscience. Ano: 2008 URL: http://precedings.nature.com/documents/1602/version/1 Changes in Cholesterol Metabolism in Peripheral Cells of Alzheimer Disease Patients and Their Relatives Nature Precedings Alessandra Pani; Paolo La Colla; Claudia Abete; Claudia Mulas; Marirosa Putzolu; Claudia Norfo; Sergio Laconi; Anna Borgia; Christina Zaru; Manuela Palmas; Paolo F. Putzu; Alessandra Mocali; Francesco Paoletti; Sandra Dessi. Background. Previous epidemiological and experimental studies indicated cholesterol as a central player in Alzheimer disease (AD). Here, we utilized skin fibroblasts and PBMC as possible ex vivo models for the study of dysfunctions of cholesterol homeostasis which may be related to AD development. 
Methods. We analyzed cholesterol homeostasis using colorimetric, thin layer chromatography (TLC), and histologic technique in ex vivo cultures of skin fibroblasts and PBMCs from patients with probable AD and their first-degree relatives. Additionally, healthy age-matched individuals served as controls. 
Findings. As compared to controls, skin fibroblasts and PBMCs from AD patients, displayed an evident alteration of cholesterol... Tipo: Manuscript Palavras-chave: Molecular Cell Biology; Neuroscience. Ano: 2007 URL: http://precedings.nature.com/documents/1246/version/1 Registros recuperados: 5 Primeira ... 1 ... Última

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