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| Coelho,Alexandre Rodrigo; Celli,Marcos Giovani; Ono,Elisabete Yurie Sataque; Wosiacki,Gilvan; Hoffmann,Fernando Leite; Pagnocca,Fernando Carlos; Hirooka,Elisa Yoko. |
| Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and > 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillium expansum; Antagonist yeasts; Biocontrol; Patulin; Degradation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400019 |
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| Portes,Ciro da Silva; Oliveira,Andriélen Virke de; Simer,Patrícia; Lunkes,Alessandra Machado; Coelho,Alexandre Rodrigo. |
| This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Moulds; Killer yeasts; Biocontrol; Antibiosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400012 |
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