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| Pereira,I.A.; Soares,L.C.; Coelho,S.M.O.; Balbino,F.A.; Pribul,B.R.; Souza,M.M.S.. |
| Avaliou-se o perfil de suscetibilidade bacteriana de diferentes sítios infecciosos frente aos antimicrobianos de eleição e determinaram-se o perfil de atividade in vitro e a concentração inibitória mínima (CIM) da azitromicina. Diferentes testes fenotípicos detectaram resistência à azitromicina em 45% de Staphylococcus spp. e 65% dos bastonetes Gram-negativo. A CIM50 para S. aureus foi 4,0μg/mL para S. intermedius 1,0μg/mL, Staphylococcus spp. coagulase-negativo >512μg/mL e bastonetes Gram-negativo 256μg/mL. Investigou-se, também, uma possível resistência cruzada entre oxacilina e azitromicina por meio da detecção do gene mecA em Staphylococcus spp. Foi possível detectar resistência à azitromicina em nove (15%) isolados de Staphylococcus spp. mecA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Gato; Resistência bacteriana; Azitromicina. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300009 |
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| Soares,B.S.; Melo,D.A.; Motta,C. C.; Marques,V.F.; Barreto,N.B.; Coelho,S.M.O.; Coelho,I.S.; Souza,M.M.S.. |
| ABSTRACT This study aims to detect the main virulence and antimicrobial resistance genes in Stapylococcus aureus from bovine mastitic milk as well as classifying them according to agr typing. A total of 55 strains from six dairy unities in the state of Rio de Janeiro were selected, of these 27.3% presented fbnA and 78,2% for fbnB genes, respectively. None of the strains tested were positive for cap5 gene, 3.6% were positive for cap8 gene. Additionally, 94.5% of strains had hlA gene and 89.1% had hlB gene while 67.3% of the strains had icaA gene and 87.3% had icaD gene. From these results it was possible to establish 12 different virulence profiles. Prevalence of agrII type was detected in 81.8% of the isolates. Concerning antimicrobial resistance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agr typing antibiotics; Bovine mastits; Staphylococcus aureus; Virulenc. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400843 |
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| Melo,D.A.; Motta,C.C.; Rojas,A.C.C. M.; Soares,B.S.; Coelho,I.S.; Coelho,S.M.O.; Souza,M.M.S.. |
| ABSTRACT This study aimed to identify Coagulase-Negative Staphylococci (CoNS) species isolated from bovine mastitis, through phenotypic and PCR-RFLP (Restriction Fragment Length Polymorphism-Polimerase Chain Reaction) methods and to compare both techniques to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique. Among them, the PCR-RFLP method, using a partially conserved sequence of the groEL gene, is a promising alternative, because of its reproducibility and reliability. On the other hand, the proteomic technique MALDI-TOF MS provides an accurate and much faster diagnosis and has been increasingly employed in microbiological identification. The pheno-genotypic profiles of beta-lactam resistance were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus spp.; Bacterial resistance; PCR-RFLP and MALDI-TOF MS. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200368 |
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