The effect of different combinations of chemical scarification, soaking and substrates (ex vitro and in vitro) on seed germination ofPolaskia chichipe (Goss.) Backeberg and Echinocactus platyacanthus Link & Otto fa. grandis (Rose) Bravo-Hollis, collected in the Tehuacan Valley, Mexico, were studied. The P chichipe seeds did not require either chemical scarification or soaking, whereas E platyacanthus required chemical scarification with strong acids. Growth and maintenance of the seedlings was evaluated in two different concentrations of Murashige-Skoog medium (50% and 100% of their components). It was found that MS 50% stimulated germination in both species and promoted seedling growth. |