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| Zaldívar,Mercedes; Velásquez,Juan Carlos; Contreras,Inés; Pérez,Luz María. |
| A mutant of the native fungus Trichoderma aureoviride, 7-121, selected for its overproduction of extracellular cellulase and ß-glucosidase (cellobiase) was obtained. In shake flask cultures, production of endoglucanase, filter paper activity and cellobiase increased two to four- fold as compared with the wild type strain. The mutant strain is stable and grows rapidly in liquid as well as in solid culture media. Enzyme yields were best when pH was controlled so that it did not fall bellow pH 3.5. Cellobiase production by this mutant is particularly high (approximately 5 U/ml) as compared to other Trichoderma, strains, which makes it a suitable candidate for waste cellulose degradation. In addition, the mutant strain showed enhanced production of fungal cell... |
| Tipo: Journal article |
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| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300011 |
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| Espinoza,Rodrigo A.; Silva‑Valenzuela,Cecilia A.; Amaya,Fernando A.; Urrutia,Ítalo M.; Contreras,Inés; Santiviago,Carlos A.. |
| Abstract Background Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages. Results Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi... |
| Tipo: Journal article |
Palavras-chave: Salmonella; Enteritidis; Typhi; SPI-13; SPI-8; Macrophages; RAW264.7; THP-1. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100402 |
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