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| Cordero,Cecilia; Cáceres,Pablo; González,Gloria; Quiroz,Karla; Carmen,Bravo; Ramírez,Ricardo; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. |
| Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`),... |
| Tipo: Journal article |
Palavras-chave: Ectomycorrhizas; Molecular markers; PCR; CAPS; Ectomicorrizas; Marcadores moleculares; PCR; CAPS. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300022 |
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| Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. |
| A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S... |
| Tipo: Journal article |
Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018 |
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| Ramírez,Claudio C.; Salazar,Marcela; Palma,R. Eduardo; Cordero,Cecilia; Meza-Basso,Luís. |
| Neotropical Rhagoletis species are arranged in four groups: nova,psalida,striatella and ferruginea, which include 18 species. On both sides of the Andes, the evolution of morphological differences among these groups has been suggested to be related to the Andes uplift process. In order to test this hypothesis, a phylogenetic analysis of morphological and molecular data was performed. The results suggest that: 1) Neotropical species of Rhagoletis constitute a separate group from Paleartic and North American species, with the only exception being a member of the striatella group having a certain association with the northern species. 2) Neotropical species seem to form a monophyletic clade, although statistical support for this is weak. 3) The split of South... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fruit fly; Diversification; Phylogeny; South América. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000600005 |
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