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Use of microsatellites for evaluation of genetic diversity in cherry tomato Bragantia
Aguirre,Nelson Ceballos; López,Walter; Orozco-Cárdenas,Martha; Coronado,Yacenia Morillo; Vallejo-Cabrera,Franco.
ABSTRACT Much of the diversity of tomato is found in wild forms, the most important being the Solanum lycopersicum L. var. cerasiforme and S. pimpinellifolium. The objective of this research was to assess the genetic diversity of 30 introductions of cherry tomato with 36 microsatellite molecular markers. The study was conducted at the Plant Transformation Research Center (PTRC) of the University of California. A dendrogram was built using the Dice-Nei and Li similarity index and the UPGMA clustering method, where introductions were differentiated without preserving a distribution pattern obeying the geographical area of origin. A coefficient of genetic differentiation was found (Fst = 0.3474), showing a high genetic differentiation of the introductions;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant genetic resources; Microsatellites; Real-time PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200220
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Genetic diversity in oil palm (Elaeis guineensis Jacq) using RAM (Random Amplified Microsatellites) Bragantia
Cardona,Christian Camilo Castañeda; Coronado,Yacenia Morillo; Conronado,Ana Cruz Morillo; Ochoa,Iván.
ABSTRACT The oil palm (Elaeis guineensis Jacq) plays an important role in the economy of some tropical countries; Colombia ranks first for production in Latin America and fifth in the world. The aim of this research was to characterize the genetic variability of 51 oil palm genotypes from the Congo with seven randomly amplified microsatellite markers RAM. As a result, 241 alleles were generated andthe number of polymorphic loci ranged from 46 to 14 for the ACA and CGA primers, respectively. High genetic diversity was found, with a total heterozygosity of 0.64, along with a high percentage of polymorphic loci, 89%. The coefficient of genetic differentiation (Fst) was 0.15, indicating that there was moderate genetic differentiation. The genetic diversity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breeding; Genetic markers; Germplasm; Genetic structure.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000400546
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