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Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53 ArchiMer
Longeon, Arlette; Peduzzi, Jean; Barthelemy, Michel; Corre, Sophie; Nicolas, Jean-louis; Guyot, Museum National D'Histoire Naturelle.
A marine bacterium, X 153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives all apparent molecular mass of 87 kDa....
Tipo: Text Palavras-chave: Probiotic bacteria; Pseudoalteromonas; Antimicrobial protein.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-426.pdf
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Utilisation des hydrocarbures par la microflore bactérienne du site hydrothermal du bassin de Guaymas (Golfe de Californie) ArchiMer
Marchand, Michel; Caprais, Jean-claude; Corre, Sophie; Jacq, Evelyne; Hussein, Dira.
Microbial utilization of occurring hydrocarbons by the sediment microbiota of Guaymas basin hydrothermal site (Gulf of California) The Guaymas Basin (Gulf of California) is an actively spreading oceanic basin and a site of hydrothermal activity. The basin floor, at about 2,000 m depth, is covered with a thick layer of pelagic sediments. The thermal alteration of recent sedimentary organic matter by the hydrothermal system generates hydrothermal petroleum. During the French-Mexican cruise Guaynaut, in November 1991, various samples were collected by means of the French submersible Nautile. Among the different scientific objectives of this campaign is the examination of the potential for hydrocarbon degradation by microbiota. Investigations were carried out...
Tipo: Text Palavras-chave: HYDROTHERMALISM; GUAYMAS; HYDROCARBONS; BIODEGRADATION; MICROBIOTA.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00091/20239/17890.pdf
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