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| Cortez,A.; Castro,A.M.G.; Heinemann,M.B.; Soares,R.M.; Leite,R.C.; Scarcelli,E.; Genovez,M.E.; Alfieri,A.A.; Richtzenhain,L.J.. |
| Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV) and bovine viral diarrhea (BVDV) in 3 animals (2.4%) each, and 1 for the association of BVDV and BHV. In 77.4 % (96/124) of the samples it was not possible to detect any agent. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Aborto; Feto; PCR; RT-PCR. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600036 |
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| Horta,M.C.; Pinter,A.; Cortez,A.; Soares,R.M.; Gennari,S.M.; Schumaker,T.T.S.; Labruna,M.B.. |
| Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rickettsia felis; Ctenocephalides felis felis; Dog; 190-kDa gene; Brazil. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300008 |
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