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Boron transport in Eucalyptus. 2. Identification in silico of a putative boron transporter for xylem loading in eucalypt Genet. Mol. Biol.
Domingues,Douglas Silva; Leite,Susi Meire Maximino; Farro,Ana Paula Cazerta; Coscrato,Virgínia Elias; Mori,Edson Seizo; Furtado,Edson Luiz; Wilcken,Carlos Frederico; Velini,Edivaldo Domingues; Guerrini,Iraë Amaral; Maia,Ivan Godoy; Marino,Celso Luis.
Boron (B) is a low mobility plant micronutrient whose molecular mechanisms of absorption and translocation are still controversial. Many factors are involved in tolerance to Boron excess or deficiency. Recently, the first protein linked to boron transport in biological systems, BOR1, was characterized in Arabidopsis thaliana. This protein is involved in boron xylem loading and is similar to bicarbonate transporters found in animals. There are indications that BOR1 is a member of a conserved protein family in plants. In this work, FORESTS database was used to identify sequences similar to this protein family, looking for a probable BOR1 homolog in eucalypt. We found five consensus sequences similar to BOR1; three of them were then used in multiple alignment...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eucalyptus; Boron; Xylem loading; Expressed Sequence Tag (EST); BOR1.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400017
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Putative metabolic pathway of mannitol and sorbitol and in sugarcane Scientia Agricola
Marino,Celso Luís; Leite,Susi Meire Maximino; Farro,Ana Paula Cazerta; Sassaki,Flávio Tetsuo; Campos,Henrique Luis Veronezi de; Coscrato,Virgínia Elias.
Until the mid 1950s, boron was believed to play an important role in the transport of sugars in plants. However, boron actually depends on sugar alcohols to be taken up by the plant. In some cases, the main sugars involved in this process are sorbitol and mannitol which form stable complexes with boron. In this study, the sequences of the SugarCane EST Genome Project (SUCEST) database were searched for enzymes involved in the metabolism of these sugars by comparing them with enzymes from other organisms. Eighteen contigs from sugarcane (Saccharum sp.) presented high similarity with 11 enzymes involved in the putative biosynthetic pathway of sorbitol and mannitol from fructose in sugarcane. Seven of these contigs had high homology with sequences deposited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Boron; Expressed sequence tags (ESTs); Polyols; Sugar alcohol.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400017
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Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) BJM
Marcon,Helena Sanches; Coscrato,Virgínia Elias; Selivon,Denise; Perondini,André Luiz Paranhos; Marino,Celso Luis.
Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anastrepha; Cross reaction; FtsZ; Wsp.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200046
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