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Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland Ciência Rural
Costa,João Carlos Miguel; Espeschit,Isis de Freitas; Pieri,Fábio Alessandro; Benjamin,Laércio Anjos; Moreira,Maria Aparecida Scatamburlo.
Bacterial biofilms are involved in the aggravation and recurrence of clinical mastitis in dairy herds. Several factors such as pH, temperature, concentration of O2 and glucose can affect their induction and growth rates. In this study, biofilm production was demonstrated by 27 Escherichia coli strains isolated from bovine mastitis at different pH values depending on the availability of glucose, mimicking conditions found in mammary glands affected by the disease. Biofilm formation was analyzed by spectrophotometric analysis in microtiter plate with 16 different culture media and by scanning electron microscopy. Biofilm formation was greater in isolates cultured under conditions associated with low glucose availability (0.5% or 1.5%) and with either an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mammary gland; Escherichia coli; Milk; Acidity; Sugar.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400015
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