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| Arns,C.W.; Campalans,J.; Costa,S.C.B.; Domingues,H.G.; D'Arce,R.C.F.; Almeida,R.S.; Coswig,L.T.. |
| This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine respiratory syncytial virus; BRSV; Virus isolation; Characterization; Nested RT-PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200008 |
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| Castro,S.M.; Sporleder,H.; Schröeder,R.; Santos,A.; Garcia,V.; Neumann,J.; Costa,S.C.B.. |
| We have determined the number of circulating T, B and natural killer cells in renal transplant recipients in order to detect changes during cytomegalovirus (CMV) infections. Serial blood samples were taken from 61 patients on standard triple immunosuppression therapy (cyclosporin A, azathioprine and prednisone). Using two-color flow cytometry analysis, the absolute number of CD3+, CD4+, CD8+, CD19+, CD3+HLA-DR+ and CD16+56+ cells was determined. Forty-eight patients (78.7%) developed active CMV infection, and all of them subsequently recovered. Twenty of the infected patients (32.8%) presented symptoms compatible with CMV disease during the infectious process. The number of lymphocytes and their main subpopulations were normal before the onset of CMV... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transplantation; Cytomegalovirus; Antigenemia; T lymphocytes. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600016 |
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