|
|
|
|
|
| Zanella,Janice; Costa,Sergio Olavo Pinto da; Zacaria,Jucimar; Echeverrigaray,Sergio. |
| Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acanthamoeba; Monocystic culture; Micromanipulation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001 |
| |
|
|
| Teixeira,Lais Helena; Rocha,Silvana; Pinto,Rosa Maria Ferreiro; Caseiro,Marcos Montani; Costa,Sergio Olavo Pinto da. |
| Acanthamoeba and Naegleria species are free-living amoebae (FLA) found in a large variety of natural habitats. The prevalence of such amoebae was determined from dust samples taken from public non-hospital internal environments with good standards of cleanliness from two campuses of the same University in the city of Santos (SP), Brazil, and where young and apparently healthy people circulate. The frequency of free-living amoebae in both campuseswas 39% and 17% respectively, with predominance of the genus Acanthamoeba. On the campus with a much larger number of circulating individuals, the observed frequency of free-living amoebae was 2.29 times larger (P< 0.00005). Two trophozoite forms of Naegleria fowleri, are the only species of this genus known to... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Free - living amoebae; Acanthamoeba spp.; Naegleria spp.; Prevalence; Dust from internal environments; Brazil. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600001 |
| |
|
| |
|
|
|
