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Magalhães,Hemerson I.F.; Ferreira,Paulo M.P.; Moura,Eraldo S.; Torres,Márcia R.; Alves,Ana P.N.N.; Pessoa,Otília D.L.; Costa-Lotufo,Letícia V.; Moraes,Manoel O.; Pessoa,Cláudia. |
The cytotoxic potential of stem organic extracts from Calotropis procera (Asclepiadaceae) was firstly evaluated against cancer cell lines by MTT assay. Subsequently, samples considered cytotoxic were tested for antimitotic activity on sea urchin egg development and for in vivo antiproliferative activity in mice bearing Sarcoma 180 tumor. Among the five extracts (hexane, dichloromethane, ethyl acetate, acetone and methanol), ethyl acetate and acetone extracts displayed higher cytotoxic potential against tumor cells, with IC50 ranging from 0.8 to 4.4 μg/mL, while methanolic extract was weakly cytotoxic. Cytotoxic extracts also exhibited cell division inhibition capacity by antimitotic assay, revealing IC50 values lower than 5 μg/mL. In the in vivo antitumor... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimitotic; Antiproliferative; Calotropis procera; Sarcoma 180 tumor; Stem extracts. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200017 |
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Ferreira,Paulo Michel P.; Farias,Davi F.; Viana,Martônio P.; Souza,Terezinha M.; Vasconcelos,Ilka M.; Soares,Bruno M.; Pessoa,Cláudia; Costa-Lotufo,Letícia V.; Moraes,Manoel O.; Carvalho,Ana F.U.. |
This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antiproliferative potential; Northeastern Brazilian plants; Myracrodruon urundeuva; Sarcoa 180 tumor; Seed extracts. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023 |
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