| Ascorbic acid or vitamin C is mostly found in natural products such as fruits and vegetables. Ultra performance liquid chromatography (UPLC) method has been developed to compare the ascorbic acid content in some fresh fruits (apple, orange) and vegetables (carrot, beet, cherry tomato) extracts with two different extraction solvents; i) 9% metaphosphoric acid, ii) 3% citric acid. The compound has been detected and quantified by the use of UPLC equipped with Photodiode Array (PDA) detector. The amount of ascorbic acid detected in fruits and vegetables extracts prepared with the two solvents mentioned was different. For the orange extracted using citric acid 3% recorded the highest concentration of ascorbic acid (38.2mg/100g FW) higher than with 9%... |
