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Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves Ciência Rural
Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
ABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451
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Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil Ciência Rural
Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos Bryan; Silva,Rodrigo Otávio Silveira.
ABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolated from diarrheal feces and sixteen (43.2%) were from the feces of non-diarrheic dogs. Enteropathogenic E. coli (EPEC) were the most frequently (62.2%) detected pathovar in dog feces and were mainly from phylogroup B1 and E. Necrotoxigenic E. coli were detected in 16.2% of the virulence-positive isolates and these contained the cytotoxic necrotizing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dogs; Escherichia coli; Pathovars; Phylogroups.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200452
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Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa Ciência Rural
Scherer,Carolina Boesel; Botoni,Larissa Silveira; Coura,Fernanda Morcatti; Silva,Rodrigo Otávio; Santos,Rafael Dantas dos; Heinemann,Marcos Bryan; Costa-Val,Adriane Pimenta.
ABSTRACT: Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria were cultured using Muller-Hinton agar (supplemented with 5% equine blood and incubated at 37°C for 24 to 48 hours. All colonies underwent biochemical evaluation for identification of staphylococci. The identity of colonies as SP was confirmed by polymerase chain reaction. The antimicrobial susceptibility of SP strains was evaluated by disk diffusion....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dogs; MRSP; Methicillin-resistant staphylococcus; Otitis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400453
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