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| Registros recuperados: 39 | |
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| Blanc, Mélanie; Antczak, Philipp; Cousin, Xavier; Grunau, Christoph; Scherbak, Nikolai; Rüegg, Joëlle; Keiter, Steffen H.. |
| The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant and can induce transgenerational effects in non-target organisms. However, associated underlying mechanisms remain unclear. The aim of this study was to investigate permethrin-related transgenerational effects in the zebrafish model, and to identify possible molecular mechanisms underlying inheritance. Zebrafish (F0) were exposed to permethrin during early-life (2 h post-fertilization up to 28 days). The F1 and F2 offspring generations were obtained by pairing exposed F0 males and females, and were bred unexposed. Locomotor and anxiety behavior were investigated, together with transcriptomic and... |
| Tipo: Text |
Palavras-chave: Transgenerational epigenetic inheritance; Developmental neurotoxicity; Glutamatergic signaling; DNA methylation; Pesticide. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00685/79661/82425.pdf |
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| Perrichon, Prescilla; Le Bihanic, Florane; Bustamante, Paco; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome; Cousin, Xavier. |
| Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals... |
| Tipo: Text |
Palavras-chave: Fish embryo-larval assay; PAHs; Artificial sediment; EROD; Behavioral performance; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35323/37905.pdf |
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| Vignet, Caroline; Trenkel, Verena; Vouillarmet, Annick; Bricca, Giampiero; Begout, Marie-laure; Cousin, Xavier. |
| Zebrafish were exposed through diet to two environmentally relevant polycyclic aromatic hydrocarbons (PAHs) mixtures of contrasted compositions, one of pyrolytic (PY) origin and one from light crude oil (LO). Monoamine concentrations were quantified in the brains of the fish after six month of exposure. A significant decrease in noradrenaline (NA) was observed in fish exposed to both mixtures, while a decrease in serotonin (5HT) and dopamine (DA) was observed only in LO-exposed fish. A decrease in metabolites of 5HT and DA was observed in fish exposed to both mixtures. Several behavioural disruptions were observed that depended on mixtures, and parallels were made with changes in monoamine concentrations. Indeed, we observed an increase in anxiety in fish... |
| Tipo: Text |
Palavras-chave: Monoamine; Polycyclic aromatic hydrocarbons; Behaviour; Anxiety; Swimming activity; Zebrafish. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00375/48578/48900.pdf |
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| Horri, Khaled; Alfonso, Sebastien; Cousin, Xavier; Munschy, Catherine; Loizeau, Veronique; Aroua, Salima; Begout, Marie-laure; Ernande, Bruno. |
| Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants that have been shown to affect fish life-history traits such as reproductive success, growth and survival. At the individual level, their toxicity and underlying mechanisms of action have been studied through experimental exposure. However, the number of experimental studies approaching marine environmental situations is scarce, i.e., in most cases, individuals are exposed to either single congeners, or single types of molecules, or high concentrations, so that results can hardly be transposed to natural populations. In the present study, we evaluated the effect of chronic dietary exposure to an environmentally realistic marine mixture of PCB and... |
| Tipo: Text |
Palavras-chave: Contaminants; Body length; Condition; Fertilization rate; Energy allocation; Trade-off. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00396/50694/51450.pdf |
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| Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome. |
| A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic... |
| Tipo: Text |
Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf |
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| Ramanoudjame, Laetitia; Rocancourt, Claire; Laine, Jeanne; Klein, Arnaud; Joassard, Lucette; Gartioux, Corine; Fleury, Marjory; Lyphout, Laura; Kabashi, Edor; Ciura, Sorana; Cousin, Xavier; Allamand, Valerie. |
| Collagen VI (COLVI), a protein ubiquitously expressed in connective tissues, is crucial for structural integrity, cellular adhesion, migration and survival. Six different genes are recognized in mammalians, encoding six COLVI-chains that assemble as two ‘short’ (α1, α2) and one ‘long’ chain (theoretically any one of α3–6). In humans, defects in the most widely expressed heterotrimer (α123), due to mutations in the COL6A1-3 genes, cause a heterogeneous group of neuromuscular disorders, collectively termed COLVI-related muscle disorders. Little is known about the function(s) of the recently described α4-6 chains and no mutations have been detected yet. In this study, we characterized two novel COLVI long chains in zebrafish that are most homologous to the... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00297/40773/46351.pdf |
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| Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier. |
| The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel... |
| Tipo: Text |
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf |
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| Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
| Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
| Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
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| Cormier, Bettie; Gambardella, Chiara; Tato, Tania; Perdriat, Quentin; Costa, Elisa; Veclin, Cloé; Le Bihanic, Florane; Grassl, Bruno; Dubocq, Florian; Kärrman, Anna; Van Arkel, Kim; Lemoine, Soazig; Lagarde, Fabienne; Morin, Benedicte; Garaventa, Francesca; Faimali, Marco; Cousin, Xavier; Bégout, Marie-laure; Beiras, Ricardo; Cachot, Jerome. |
| Microplastics are ubiquitous in aquatic ecosystems, but little information is currently available on the dangers and risks to living organisms. In order to assess the ecotoxicity of environmental microplastics (MPs), samples were collected from the beaches of two islands in the Guadeloupe archipelago, Petit-Bourg (PB) located on the main island of Guadeloupe and Marie-Galante (MG) on the second island of the archipelago. These samples have a similar polymer composition with mainly polyethylene (PE) and polypropylene (PP). However, these two samples are very dissimilar with regard to their contamination profile and their toxicity. MPs from MG contain more lead, cadmium and organochlorine compounds while those from PB have higher levels of copper, zinc and... |
| Tipo: Text |
Palavras-chave: Environmental microplastics; Leachates; Early life stages; Aquatic organisms; Toxicity. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00661/77276/78711.pdf |
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| Alfonso, Sebastien; Sadoul, Bastien; Cousin, Xavier; Bégout, Marie-laure. |
| In aquaculture, fish are exposed to unavoidable stressors that can be detrimental for their health and welfare. However, welfare in farmed fish can be difficult to assess, and, so far, no standardized test has been universally accepted as a welfare indicator. This work contributes to the establishment of behavioural welfare indicators in a marine teleost in response to different water quality acute stressors. Groups of ten fish were exposed to high Total Ammonia Nitrogen concentration (High TAN, 18 mg.L-1), Hyperoxia (200 % O2 saturation), Hypoxia (20 % O2 saturation), or control water quality (100% O2 saturation and TAN < 2.5 mg.L-1) over 1 hour. Fish were then transferred in a novel environment for a group behaviour test under the same water quality... |
| Tipo: Text |
Palavras-chave: Fish; Welfare; Water quality; Behaviour; Stress. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00612/72399/71344.pdf |
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| Carballo, Carlos; Berbel, Concha; Guerrero-cozar, Israel; Jimenez-fernandez, Eduardo; Cousin, Xavier; Begout, Marie-laure; Manchado, Manuel. |
| Internal electronic tagging is a major issue in flatfish species due to the small size of abdominal cavity. In this study, three tag types, referred to as Nonatec, nano and mini, were evaluated in three weight classes of Senegalese sole: small (0.3 g), middle (0.8 g) and large (2.0 g). Tags were injected from the blind side and fish were carefully handled to minimize sharp movements. Tag losses were 8% in the small size class, between 5.2 and 15.1% in the middle size class and 2–4% in the large size class. The mortality rates ranged between 2.0 and 15.0% with the lowest values in the large size class. No negative effects of tags on growth (tagged vs non-tagged fish using a middle size class) were found after 57 days of culture. Four additional trials using... |
| Tipo: Text |
Palavras-chave: Flatfish; PIT-tags; Loss rates; Sex effect; Growth. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00439/55090/56532.pdf |
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| Beiras, R; Bellas, J.; Cachot, J; Cormier, B.; Cousin, Xavier; Engwall, M.; Gambardella, C.; Garaventa, F.; Keiter, S.; Le Bihanic, F.; Lopez-ibanez, S.; Piazza, V.; Rial, D.; Tato, T.; Vidal-linan, L.. |
| Toxicity of polyethylene microplastics (PE-MP) of size ranges similar to their natural food to zooplanktonic organisms representative of the main taxa present in marine plankton, including rotifers, copepods, bivalves, echinoderms and fish, was evaluated. Early life stages (ELS) were prioritized as testing models in order to maximize sensitivity. Treatments included particles spiked with benzophenone-3 (BP-3), a hydrophobic organic chemical used in cosmetics with direct input in coastal areas. Despite documented ingestion of both virgin and BP-3 spiked microplastics no acute toxicity was found at loads orders of magnitude above environmentally relevant concentrations on any of the invertebrate models. In fish tests some effects, including premature or... |
| Tipo: Text |
Palavras-chave: Polyethylene; Marine litter; Marine zooplankton; Benzophenone-3; Embryo-larval bioassays. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00452/56369/57965.pdf |
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| Daouk, Tarek; Larcher, Thibaut; Roupsard, Francois; Lyphout, Laura; Rigaud, Cyril; Ledevin, Mireille; Loizeau, Veronique; Cousin, Xavier. |
| Although the use of polychlorinated biphenyls (PCBs) has been banned for several decades, they are still present in the environment and are occasionally mechanically released from sediment or transferred through the trophic chain. Field analyses have established correlations between exposure to PCBs and alterations in fish physiology including reproductive function. Experimental exposures have been mainly performed using dioxin-like PCBs or other congeners at very high concentrations. However, these studies are often difficult to relate to real-life conditions. In the present study, we performed a life-cycle exposure using zebrafish model and mixtures representative of some environmental situations in terms of doses, composition and containing mainly non... |
| Tipo: Text |
Palavras-chave: Zebrafish; PCBs; Chronic exposure; Physiology; Reproduction; Atresia. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00059/17038/14608.pdf |
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| Vignet, Caroline; Devier, Marie-hélène; Le Menach, Karyn; Lyphout, Laura; Potier, Jeremy; Cachot, Jerome; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier. |
| A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g−1 which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of... |
| Tipo: Text |
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Lifecycle; Anxiety-like behavior; Offspring. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29909/28497.pdf |
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| Vandeputte, Marc; Clota, Frederic; Sadoul, Bastien; Blanc, Marie-odile; Blondeau-bidet, Eva; Bégout, Marie-laure; Cousin, Xavier; Geffroy, Benjamin. |
| Temperature‐dependent sex determination (TSD) can be observed in multiple reptile and fish species. It is adaptive when varying environmental conditions advantage either males or females. A good knowledge of the thermosensitive period is key to understand how environmental changes may lead to changes in population sex ratio. Here, by manipulating temperature during development, we confirm that cold temperature (16°C) increases the proportion of fish that develop as females in European sea bass (Dicentrarchus labrax) until 56 days posthatching, but show that it has an opposite effect at later stages, with the proportion of males reaching ~90% after 230 days at 16°C. This is the first observation of opposite effects of temperature at different time periods... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Fish; Sex ratio; Temperature-dependent sex determination; Thermosensitive period; TSD. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/76965/78184.pdf |
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| Perrichon, Prescilla; Akcha, Farida; Le Menach, Karyn; Goubeau, Manon; Budzinski, Helene; Cousin, Xavier; Bustamante, Paco. |
| In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3 ×, 1 × and 3 ×; 1 × represents an environmental concentration measured in French estuary).... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbons; Parental transmission; Fish early-life stages; PhotoMotor Response; Cardiac performance; PAH metabolites; Genotoxicity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00259/37046/37174.pdf |
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| Armesto, Paula; Infante, Carlos; Cousin, Xavier; Ponce, Marian; Manchado, Manuel. |
| In the present work, seven genes encoding Na+,K+-ATPase (NKA) β-subunits in the teleost Solea senegalensis are described for the first time. Sequence analysis of the predicted polypeptides revealed a high degree of conservation with those of other vertebrate species and maintenance of important motifs involved in structure and function. Phylogenetic analysis clustered the seven genes into four main clades: β1 (atp1b1a and atp1b1b), β2 (atp1b2a and atp1b2b), β3 (atp1b3a and atp1b3b) and β4 (atp1b4). In juveniles, all paralogous transcripts were detected in the nine tissues examined albeit with different expression patterns. The most ubiquitous expressed gene was atp1b1a whereas atp1b1b was mainly detected in osmoregulatory organs (gill, kidney and... |
| Tipo: Text |
Palavras-chave: Senegalese sole Na+; K+-ATPase Beta subunit Paralogs Osmoregulation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00240/35173/35782.pdf |
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| Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different... |
| Tipo: Text |
Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf |
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| Armesto, Paula; Campinho, Marco A.; Rodríguez-rúa, Ana; Cousin, Xavier; Power, Deborah M.; Manchado, Manuel; Infante, Carlos. |
| In the present work, five genes encoding different Na+,K+ ATPase (NKA) α-isoforms in the teleost Solea senegalensis are described for the first time. Sequence analysis of predicted polypeptides revealed a high degree of conservation across teleosts and mammals. Phylogenetic analysis clustered the five genes into three main clades: α1 (designated atp1a1a and atp1a1b), α2 (designated atp1a2) and α3 (designated atp1a3a and atp1a3b) isoforms. Transcriptional analysis in larvae showed distinct expression profiles during development. In juvenile tissues, the atp1a1a gene was highly expressed in osmoregulatory organs, atp1a2 in skeletal muscle, atp1a1b in brain and heart and atp1a3a and atp1a3b mainly in brain. Quantification of mRNA abundance after a salinity... |
| Tipo: Text |
Palavras-chave: Senegalese sole Na+; K+ ATPase atp1a1a atp1a1b atp1a2 atp1a3a atp1a3b osmoregulation. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00195/30640/29093.pdf |
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| Registros recuperados: 39 | |
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