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Application of microsatellite markers for breeding and genetic conservation of herds of Pantaneiro sheep Electron. J. Biotechnol.
Crispim,Bruno do Amaral; Seno,Leonardo de Oliveira; Egito,Andréa Alves do; Vargas Junior,Fernando Miranda de; Grisolia,Alexéia Barufatti.
Background The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate. A total of 127 animals were genotyped at eight microsatellite loci. They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil). Results The population of Pantaneiro sheep from the UFGD exhibited a high mean number of alleles (11.13) and allelic richness (10.66). The polymorphic information content was highly informative in the locus studied, resulting in a mean value of 0.71. Observed heterozygosity was lower than...
Tipo: Journal article Palavras-chave: Animal genetic resources; Genetic diversity; Kinship; Locally adapted breed; STR.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600010
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Toxicological properties of an aqueous extract of Aristolochia triangularis leaves, using the brine shrimp lethality and Allium cepa bioassays Ciência Rural
Silva,José Darlan Alves da; Nogueira,Cláudio Rodrigo; Vieira,Maria do Carmo; Heredia-Vieira,Silvia Cristina; Barufatti,Alexeia; Crispim,Bruno do Amaral; Francisco,Luiza Flávia Veiga; Viana,Lucilene Finoto; Cardoso,Claudia Andrea Lima.
ABSTRACT: The aqueous extract of Aristolochia triangularis leaves showed activity against Artemia salina larvae, with an LD50 of 370.6 µg/mL. In experiments with Allium cepa seeds, the extract caused a 51.26% reduction in the germination index, inhibited mean root growth, and was cytotoxic at concentrations of 668 and 2,000 µg/mL. Its antioxidant activity was additionally assessed in this research and the ferric reducing antioxidant power value was found to be 391.2 µM/g. Such health-beneficial property can be attributed partly to the total phenolic content, spectrophotometrically determined as 52.67 mg/g. The occurrence of cytotoxicity suggested caution when consuming teas from A. triangularis leaves for medicinal purposes, and equally reveals the need...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cipó-mil-homens; Infusion; Toxicity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800753
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