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| Norris, Karl; Mishukova, Oksana; Zykwinska, Agata; Colliec-jouault, Sylvia; Sinquin, Corinne; Koptioug, Andrei; Cuenot, Stéphane; Kerns, Jemma; Surmeneva, Maria; Surmenev, Roman; Douglas, Timothy. |
| Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of... |
| Tipo: Text |
Palavras-chave: Marine exopolysaccharide; Collagen; Surface modification; Ti6Al4V. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00477/58881/61433.pdf |
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| Pigné, Sandrine; Zykwinska, Agata; Janod, Etienne; Cuenot, Stéphane; Kerkoud, Mohammed; Raulo, Roxane; Bataillé-simoneau, Nelly; Marchi, Muriel; Kwasiborski, Anthony; N’guyen, Guillaume; Mabilleau, Guillaume; Simoneau, Philippe; Guillemette, Thomas. |
| Background: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Äabmak1 strain... |
| Tipo: Text |
Palavras-chave: Monooxygenase; Cell wall; Melanin; Fungus; Flavoprotein. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48189/48305.pdf |
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