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	Provedor de dados Biocell (1) Electron. J. Biotechnol. (1) Autor Curvetto,Néstor (2) Marinangeli,Pablo (2) Deluchi,Bernardo (1) Franzone,Pascual (1) Galmarini,Claudio (1) Mockel,Gabriela (1) Rosselló,Fernando (1) Ríos,Raúl (1) Mais... Palavra-chave Agrobacterium tumefaciens (1) Allium cepa L. (1) Callus (1) Chemical sterilization (1) In vitro multiplication (1) Lily (1) Tissue culture (1) Zygotic embryos (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2006 (2) País Argentina (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Towards genetic transformation of local onion varieties Electron. J. Biotechnol. Marinangeli,Pablo; Deluchi,Bernardo; Ríos,Raúl; Franzone,Pascual; Galmarini,Claudio; Rosselló,Fernando; Curvetto,Néstor. The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of 'Valenciana' onion, Torrentina, Cobriza INTA and Grano de oro were in vitro cultivated for callus induction. After three to four months an average of 57.4% success for the three varieties was reached. Transformation was carried out with Agl1 or LBA 4404 A. tumefaciens strains, both carrying a binary vector containing the marker gene gus a and the selection gene nptII. Selection was done in callus induction medium containing 10 mgL-1 geneticin during three... Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Allium cepa L.; Callus; Zygotic embryos. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300030 Hydrogen peroxide in micropropagation of Lilium: A comparison with a traditional methodology Biocell Curvetto,Néstor; Marinangeli,Pablo; Mockel,Gabriela. The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits in all treatments, though it was higher in the H2O2... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical sterilization; Lily; In vitro multiplication; Tissue culture. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300010 Registros recuperados: 2 Primeira ... 1 ... Última

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