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| Ortega,Priscila; Santos,Rudney A.; Lacouth,Patrícia; Rozas,Enrique E.; Custódio,Márcio R.; Zanotto,Flavia Pinheiro. |
| Ucides cordatus (Linnaeus, 1763) is a hypo-hyper-regulating mangrove crab possessing gills for respiratory and osmoregulatory processes, separated in anterior and posterior sections. They also have hepatopancreas, which is responsible for digestion and absorption of nutrients and detoxification of toxic metals. Each of these organs has specific cells that are important for in vitro studies in cell biology, ion and toxic metals transport. In order to study and characterize cells from gills and hepatopancreas, both were separated using a Sucrose Gradient (SG) from 10 to 40% and cells in each gradient were characterized using the vital mitochondrial dye DASPEI (2-(4-dimethylaminostyryl)-N- ethylpyridinium iodide) and Trichrome Mallory's stain. Both in 20 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mangrove crab; DASPEI; Cell cadmium transport; In vitro cells. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212014000300011 |
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| Rozas,Enrique E.; Albano,Rodolpho M.; Lôbo-Hajdu,Gisele; Müller,Werner E.G.; Schröder,Heinz-C.; Custódio,Márcio R.. |
| Despite the large number of reports describing sponge-microbe associations, limited knowledge is available about associated fungi and their relationships with the hosts. In this work, specific fungal strains were obtained directly from in vitro sponge cell cultures (primmorphs) and single sponge cells (cytospins) and compared with those obtained from whole tissue preparations. A total of 27 fungal strains were isolated from the marine sponges Hymeniacidon heliophila and Haliclona melana. Fifteen strains, nine from H. heliophila and six from H. melana, were obtained from whole tissue and were considered as possible mesohyl associated or transient fungi. Twelve strains were isolated from in vitro sponge cell cultures (primmorphs) and were, therefore,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marine sponges; Primmorphs; Cytospins; Fungal detection. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400043 |
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