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Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation Biol. Res.
FERNÁNDEZ,VIRGINIA; MASSA,LAURA; QUIÑONES,LUIS; SIMON-GIAVAROTTI,KARIN A; GIAVAROTTI,LEANDRO; D'ALMEIDA,VÂNIA; AZZALIS,LIGIA A; JUNQUEIRA,VIRGINIA BC; VIDELA,LUIS A.
Liver microsomal cytochrome P4502E1-dependent p-nitrophenol (PNP) hydroxylation and expression of cytochrome P4502E1 were studied in rats subjected to g-hexachlorocyclohexane (HCCH) or L-3,3,,5-triiodothyronine (T3) administration as a possible mechanism contributing to superoxide radical (O2.-) generation. HCCH treatment (a single dose of 40 mg/kg body wt) produced a 43% increase in the content of total cytochrome P450, whereas T3 (daily doses of 0.1 mg/kg body wt for two consecutive days) led to a 37% decrease. NADPH-dependent O2.- generation was elevated by HCCH and T3, expressed as either per mg of protein or per nmol of cytochrome P450, with a 135% enhancement in the O2.- production/superoxide dismutase (SOD) activity ratios being observed in both...
Tipo: Journal article Palavras-chave: G-Hexachlorocyclohexane L-3; 3'; 5-Triiodothyronine Superoxide radical Cytochrome P4502E1 Rat liver.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300007
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Effects of isoflavone on oxidative stress parameters and homocysteine in postmenopausal women complaining of insomnia Biol. Res.
BRANDÃO,LETÍCIA C; HACHUL,HELENA; BITTENCOURT,LIA R; BARACAT,EDMUND C; TUFIK,SERGIO; D'ALMEIDA,VÂNIA.
Sleep disorders have an increased incidence after menopause. The objective of this work was to evaluate the effects of isoflavone on some oxidative stress markers in postmenopausal women complaining of insomnia. Women aged between 50-65 years (n=38) were recruited and assigned to a double-blind placebo controlled study for 4 months. The treated group received 100 mg/day of isoflavones. Blood collections were conducted on three different occasions to assess total glutathione; superoxide dismutase and catalase in erythrocytes; lipid peroxidation; and homocysteine plasma concentrations. No differences between the groups were observed. However, all the patients seem to improve their oxidative stress status and homocysteine concentration after treatment....
Tipo: Journal article Palavras-chave: Homocysteine; Isoflavones; Insomnia; Menopause; Oxidative stress.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300002
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