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| LEMUS,DAVID; DABANCENS,ALFREDO; ILLANES,JULIO; FUENZALIDA,MARCELA; GUERRERO,ANIBAL; LÓPEZ,CLAUDIA. |
| Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: ß-MAAC). Methylcellulose discs instilled with 10 µl of ß-MAAC (0.08 µg/ml),10 µl of tumor supernatant(TA3ts),5 µl ß-MAAC + 5 µl TA3ts, and 10 µl of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 µm2 ). The results show that ß-MAAC produced a significant... |
| Tipo: Journal article |
Palavras-chave: Antiangiogenesis; Betamethasone; CAM; Tumor. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300010 |
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| ZÚÑIGA,JORGE; FUENZALIDA,MARCELA; GUERRERO,ANÍBAL; ILLANES,JULIO; DABANCENS,ALFREDO; DÍAZ,EUGENIA; LEMUS,DAVID. |
| Angiogenesis, the development of new blood vessels from the existing vascular network, may result as a consequence of the increase or decrease of proangiogenic or antiangiogenic factors, respectively. The tumor itself could up-regulate the production of angiogenic factors. Recently, we established that the steroidal drug betamethasone in low concentration inhibit the neovascularization promoted by TA3 Ts on CAM of chick embryos. We describe here the effects of the non-steroidal drug ketoprofen, alone or in association with betamethasone, on the angiogenesis promoted by TA3 Ts on CAM. The main finding reported here is that the formation of new blood vessels is strongly inhibited by low concentrations of ketoprofen. The association of both drugs produced a... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Antiangiogenesis; Tumor; Betamethasone; Ketoprofen. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200013 |
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| ILLANES,JULIO; DABANCENS,ALFREDO; ACUÑA,OLGA; FUENZALIDA,MARCELA; GUERRERO,ANIBAL; LOPEZ,CLAUDIA; LEMUS,DAVID. |
| In this study, we showed the effect of the betamethasone, sulindac and quinacrine alone or combined, on the inflammatory angiogenesis promoted by polyurethane sponge on mice. The main finding reported here is that the formation of new blood vessels was strongly inhibited by low concentration of betamethasone, sulindac or quinacrine, whether alone or in combination. It is known that steroidal anti-inflammatory drugs inhibit the enzymes required for the production of prostaglandins through a nuclear glucocorticoid receptor (GR) mediated mechanism. This mechanism may occur in endothelial cells as well. Considering that activity of cyclo-oxigenases 1 and 2 is inhibited by sulindac, and that these enzymes are located in the stromal tissue, we propose that the... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Antiangiogenesis; Betamethasone; Sulindac; Quinacrine. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300008 |
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