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Evaluation of chromogenic media and seminested PCR in the identification of Candida species BJM
Daef,Enas; Moharram,Ahmed; Eldin,Salwa Seif; Elsherbiny,Nahla; Mohammed,Mona.
Identification of Candida cultured from various clinical specimens to the species level is increasingly necessary for clinical laboratories. Although sn PCR identifies the species within hours but its cost-effectiveness is to be considered. So there is always a need for media which help in the isolation and identification at the species level. The study aimed to evaluate the performance of different chromogenic media and to compare the effectiveness of the traditional phenotypic methods vs. seminested polymerase chain reaction (sn PCR) for identification of Candida species. One hundred and twenty seven Candida strains isolated from various clinical specimens were identified by conventional methods, four different chromogenic media and sn PCR. HiCrome...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromogenic media; Sn PCR; Candida.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100037
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