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	Provedor de dados BJMBR (2) Autor Dai,Jinhua (2) Chen,Guofang (1) Ge,Zhibin (1) Liao,Yufeng (1) Luo,Xianhai (1) Ma,Jianbo (1) Zhang,Haijun (1) Zheng,Xingguo (1) Mais... Palavra-chave Apoptosis (1) Autophagy (1) Bone fracture (1) MEK/ERK pathway (1) MicroRNA-221 (1) Myocardial infarction (1) Osteoblast differentiation (1) PI3K/AKT/mTOR pathway (1) Smad (1) Wnt/Notch (1) ZFPM2 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2018 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Polydatin protects H9c2 cells from hypoxia-induced injury via up-regulating long non-coding RNA DGCR5 BJMBR Dai,Jinhua; Ma,Jianbo; Liao,Yufeng; Luo,Xianhai; Chen,Guofang. Polydatin (PD), a monocrystalline polyphenolic drug mainly found in the roots of Polygonum cuspidatum, has various pharmacological activities. Long non-coding RNAs (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) was found to participate in the suppression of multiple cancers. Here, we proposed to study the effect of PD on myocardial infarction (MI) by inducing DGCR5. CCK-8 assay was performed to detect the viability of H9c2 cells. Flow cytometry was utilized to test apoptosis of H9c2 cells. These results determined the optimal concentration and effect time of hypoxia as well as PD. Si-DGCR5 was transfected into cells and the expression level was determined by qRT-PCR. Western blot was utilized to evaluate the expression of apoptosis-related... Tipo: Info:eu-repo/semantics/article Palavras-chave: Myocardial infarction; Apoptosis; Autophagy; PI3K/AKT/mTOR pathway; MEK/ERK pathway. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200613 MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts BJMBR Zheng,Xingguo; Dai,Jinhua; Zhang,Haijun; Ge,Zhibin. Bone fracture is a common medical condition, which may occur due to traumatic injury or disease-related conditions. Evidence suggests that microRNAs (miRNAs) can regulate osteoblast differentiation and function. In this study, we explored the effects and mechanism of miR-221 on the growth and migration of osteoblasts using MC3T3-E1 cells. The expression levels of miR-221 in the different groups were measured by qRT-PCR. Then, miR-221 mimic and inhibitor were transfected into MC3T3-E1 cells, and cell viability and migration were measured using the CCK-8 assay and the Transwell migration assay. Additionally, the expression levels of differentiation-related factors (Runx2 and Ocn) and ZFPM2 were measured by qRT-PCR. Western blot was used to measure the... Tipo: Info:eu-repo/semantics/article Palavras-chave: MicroRNA-221; Bone fracture; Osteoblast differentiation; ZFPM2; Wnt/Notch; Smad. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200602 Registros recuperados: 2 Primeira ... 1 ... Última

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