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| Damaso,Mônica Caramez Triches; Terzi,Selma da Costa; Farias,Antonio Xavier; Oliveira,Ana Carolina Pereira de; Fraga,Marcelo Elias; Couri,Sonia. |
| The aim of the present work was to select filamentous fungi isolated from diverse substrates to obtain the strains with potential to produce the hydrolytic enzymes. From a total of 215 strains, seven strains from the soils, six from the plants and one from sugarcane bagasse were selected and identified as belonging to the Trichoderma, Penicillium and Aspergillus genera. The best hydrolytic activities obtained by semi-solid fermentation using these strains were approximately: 35; 1; 160; 170 and 120 U/gdm (CMCase, FPase, β-glucosidase, xylanase and polygalacturonase, respectively), demonstrating their potential to synthesize the enzymes compared with the results reported in the literature. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400005 |
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| Damaso,Mônica Caramez Triches; Passianoto,Moisés Augusto; Freitas,Sidinéa Cordeiro de; Freire,Denise Maria Guimarães; Lago,Regina Celi Araujo; Couri,Sonia. |
| The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipase; Solid state fermentation; Agroindustrial residues; Corn oil by-products. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400015 |
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| Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei. |
| In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
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