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Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains BJM
Ferraz,Priscila N.; Danelli,Maria das Graças M..
Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma gallisepticum; Phenotypic variation; Antigenic variation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011
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Oral administration of piperine for the control of aflatoxin intoxication in rats BJM
Gagini,Thalita B.; Silva,Robson E.; Castro,Isabela S.; Soares,Breno A.; Lima,Marco E.F.; Brito,Marilene F.; Mazur,Carlos; Direito,Glória M.; Danelli,Maria das Graças M..
Aflatoxins are mycotoxins that have important toxic effects on human and animal health, even if consumed at low doses. The oral administration of piperine (1.12 mg/kg) during 23 days in rats seemingly interfered with the toxicity of aflatoxins, decreasing hepatic injuries and the leukocyte depletion in experimentally intoxicated animals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Piperine; Experimental intoxication; Rats.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200013
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Protein profile analysis by SDS-PAGE of Mycoplasma Gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media BJM
Ferraz,Priscila N.; Menezes,Claudio C. P.; Danelli,Maria das Graças M.; Lizeu,Jackeline de O. P.; Nascimento,Elmiro R. do; Lucchesi,Daniela M..
The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mycoplasma gallisepticum; SDS-PAGE; Protein profile.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200010
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