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Use of low density polyethylene membranes for assessment of genotoxicity of PAHs in the Seine River ArchiMer
Vincent-hubert, Francoise; Uher, Emmanuelle; Di Giorgio, Carole; Michel, Cecile; De Meo, Michel; Gourlay-france, Catherine.
The genotoxicity of river water dissolved contaminants is usually estimated after grab sampling of river water. Water contamination can now be obtained with passive samplers that allow a time-integrated sampling of contaminants. Since it was verified that low density polyethylene membranes (LDPE) accumulate labile hydrophobic compounds, their use was proposed as a passive sampler. This study was designed to test the applicability of passive sampling for combined chemical and genotoxicity measurements. The LDPE extracts were tested with the umu test (TA1535/pSK1002 ± S9) and the Ames assay (TA98, TA100 and YG1041 ± S9). We describe here this new protocol and its application in two field studies on four sites of the Seine River. Field LDPE extracts were...
Tipo: Text Palavras-chave: Genotoxicity; River water; Passive samplers; PAHs; Ames assay; Umu test.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48225/48402.pdf
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