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	Provedor de dados BJID (1) BJM (1) Ciência Rural (1) Electron. J. Biotechnol. (1) Autor Delamare,Ana Paula Longaray (4) Echeverrigaray,Sergio (4) Costa,Sérgio Olavo Pinto da (2) Zacaria,Jucimar (2) Artico,Liane de Oliveira (1) Gal,Orit (1) Grazziotin,Felipe Gobbi (1) Hillel,Jossi (1) Lavi,Uri (1) Michelim,Lessandra (1) Muller,Gabriela (1) Tosello,Geraldo (1) Tozoni,Daniela (1) Vanderlinde,Regina (1) Mais... Palavra-chave Molecular markers (2) Aeromonas (1) Biodegradation (1) Citronellol catabolism (1) Fingerprinting (1) Genetic distances (1) Molecular identification (1) Monoterpenes degradation (1) P. mendocina (1) PCR (1) Proteus mirabilis (1) Species identification (1) Zea mays (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (1) Ano 2010 (1) 2008 (1) 2002 (1) 1996 (1) País Brazil (3) Chile (1) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates BJID Michelim,Lessandra; Muller,Gabriela; Zacaria,Jucimar; Delamare,Ana Paula Longaray; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sergio. Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR... Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteus mirabilis; Molecular markers; Fingerprinting; PCR. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014 Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level BJM Delamare,Ana Paula Longaray; Artico,Liane de Oliveira; Grazziotin,Felipe Gobbi; Echeverrigaray,Sergio; Costa,Sérgio Olavo Pinto da. Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Molecular markers; Species identification. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016 Evaluation of the genetic diversity among maize lines (Zea mays L.) by DNA fingerprinting analysis Ciência Rural Echeverrigaray,Sergio; Delamare,Ana Paula Longaray; Tosello,Geraldo; Gal,Orit; Hillel,Jossi; Lavi,Uri. Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimate the genetic distances between them. The relationship obtained by DNA fingerprinting analysis of the eight inbreed lines was highly consistem with the ir genetical origin. Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; Genetic distances; Zea mays. Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000300028 Degradation of citronellol, citronellal and citronellyl acetate by Pseudomonas mendocina IBPse 105 Electron. J. Biotechnol. Tozoni,Daniela; Zacaria,Jucimar; Vanderlinde,Regina; Delamare,Ana Paula Longaray; Echeverrigaray,Sergio. The purpose of this work was to stud the biodegradation of citronellol, citronellal and citronellyl acetate by a soil Pseudomonas mendocina strain (IBPse 105) isolated from a Cymbopogon windelandi field. This strain efficiently used citronellol, citronellal, citronellyl acetate and myrcene as sole source of carbon, but was not able to grow on other 15 monoterpenoids evaluated. Gas chromatography-mass spectrometry (GC-MS) analysis of metabolites accumulation during P. medocina IBPse 105 growth on citronellol showed that this strain uses the citronellol catabolic pathway described for other species of the genus. IBPse 105 degradation of citronellyl acetate initiates by its hydrolysis to citronellol. The mini-Tn5 insertion in mutant IBPse 105-303, impaired in... Tipo: Journal article Palavras-chave: Biodegradation; Citronellol catabolism; Monoterpenes degradation; P. mendocina. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200002 Registros recuperados: 4 Primeira ... 1 ... Última

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