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| Mosbah, Amor; Delavenne, Emilie; Souissi, Yasmine; Mahjoubi, Mouna; Jehan, Philippe; Le Yondre, Nicolas; Cherif, Ameur; Bondon, Arnaud; Mounier, Jerome; Baudy-floc'H, Michele; Le Blay, Gwenaelle. |
| Lactobacillus harbinensis K.V9.3.1Np was described as endowed with high antifungal activity. Most of the studies associated this activity to the produced organic acids, i.e., lactic acid, acetic acid, and hexanoic acid. The aim of this study was to purify and identify, other not yet described, antifungal molecules produced by L. harbinensis K.V9.3.1 Np when used in yogurt fermentation. Active compounds were extracted through several extraction processes using organic solvents and protein precipitation. The fractions of interest were purified using flash chromatography and preparative HPLC for specific characterization. The bioactive compounds identification was performed using Nuclear Magnetic Resonance and Mass Spectrometry. Activity tests against... |
| Tipo: Text |
Palavras-chave: Spermine; Polylactates; Lactobacillus; Extraction; Purification; Ms; Nmr. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00616/72773/72221.pdf |
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| Delavenne, Emilie; Cliquet, Sophie; Trunet, Clément; Barbier, Georges; Mounier, Jérome; Le Blay, Gwenaelle. |
| Few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. Consumer’s demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. In this study, using a 25-1 factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt. L. harbinensis K.V9.3.1Np, the most relevant strain with regard to antifungal activity was... |
| Tipo: Text |
Palavras-chave: Lactobacillus harbinensis; Bioprotective culture; Antifungal activity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00189/30025/28510.pdf |
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