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Detection and molecular characterization of β-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador BJID
Delgado,Diana Yessenia Calva; Barrigas,Zorayda Patricia Toledo; Astutillo,Sofía Genoveva Ochoa; Jaramillo,Ana Paulina Arévalo; Ausili,Alessio.
ABSTRACT This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of β-lactamases and ESBLs with blaTEM and blaCTX-M as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different β-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 β-lactamase and the presence of different CTX-M variants with a prevalence of CTX-M-15. Two infrequent CTX-M variants in South America were also identified. To the best of our knowledge, this is one of the first studies describing the genetic characteristics of β-lactamases in Ecuador.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Β-Lactam resistance; Β-Lactamase; ESBL; Genotypic characterization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600627
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