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	Provedor de dados 55 (1) 58 (1) Autor Dellagostin,Odir (2) Borsuk,Sibele (1) Bourhy,Pascale (1) Hartskeerl,Rudy (1) Kremer,Frederico (1) Lilenbaum,Walter (1) McFadden,Johnjoe (1) Mendum,Tom (1) Ramos,Daniela Fernandes (1) Ristow,Paula (1) Seixas,Fabiana Kommling (1) Vasconcellos,Silvio (1) Mais... Palavra-chave BCG vaccine (1) Clinical diagnosis (1) Gene knockout techniques (1) Leptospira (1) PFGE (1) Rrs (1) Sequencing (1) Serogrouping (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2014 (1) 2012 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Rational design of diagnostic and vaccination strategies for tuberculosis 55 Borsuk,Sibele; Seixas,Fabiana Kommling; Ramos,Daniela Fernandes; Mendum,Tom; McFadden,Johnjoe; Dellagostin,Odir. The development of diagnostic tests which can readily differentiate between vaccinated and tuberculosis-infected individuals is crucial for the wider utilization of bacillus Calmette-Guérin (BCG) as vaccine in humans and animals. BCG_0092 is an antigen that elicits specific delayed type hypersensitivity reactions similar in size and morphological aspects to that elicited by purified protein derivative, in both animals and humans infected with the tubercle bacilli. We carried out bioinformatics analyses of the BCG_0092 and designed a diagnostic test by using the predicted MHC class I epitopes. In addition, we performed a knockout of this gene by homologous recombination in the BCG vaccine strain to allow differentiation of vaccinated from infected... Tipo: Info:eu-repo/semantics/other Palavras-chave: Gene knockout techniques; Clinical diagnosis; BCG vaccine. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100012 Molecular characterization of the first leptospires isolated from goats in Brazil 58 Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio. Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050 Registros recuperados: 2 Primeira ... 1 ... Última

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