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| Yu,Guangchao; Chen,Lei; Lin,Chii-wann; Li,Bing; Cui,Hemiao; Chen,Siyi; Miao,Jian; Bian,Huawei; Chen,Dingqiang; Deng,Yang. |
| BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082... |
| Tipo: Journal article |
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Integron screening; Bacterial integrons; Class 1 integron; Class 2 integron; Class 3 integron. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100076 |
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| Ma,Yanlin; Deng,Yang; Xu,Zhenbo; Liu,Junyan; Dong,Jianjun; Yin,Hua; Yu,Junhong; Chang,Zongming; Wang,Dongfeng. |
| ABSTRACT The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer. The results suggested that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propidium monoazide; Polymerase chain reaction; Beer spoilage bacteria; Lactobacillus brevis horA. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400740 |
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