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	Provedor de dados Electron. J. Biotechnol. (2) Autor Deshpande,Santosh (2) De Villiers,Santie (1) De Villiers,Santie M (1) Jones,Richard (1) Manyasa,Eric O (1) Michael,Vincent N (1) Njung'e,Vincent (1) Saiyiorri,Annis N (1) Siambi,Moses (1) Silim,Said (1) Mais... Palavra-chave Genotyping (2) Diversity analysis (1) Genetic variation (1) Microsatellites (1) SSR markers (1) Seed purity (1) Mais... Tipo do documento Journal article (2) Ano 2016 (1) 2015 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana) Electron. J. Biotechnol. De Villiers,Santie M; Michael,Vincent N; Manyasa,Eric O; Saiyiorri,Annis N; Deshpande,Santosh. Background Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3) polymorphism and ability to discern genetic diversity within the tested finger millet germplasm. Results Across 88 samples, the 52... Tipo: Journal article Palavras-chave: Diversity analysis; SSR markers; Genotyping. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200003 SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints Electron. J. Biotechnol. Njung'e,Vincent; Deshpande,Santosh; Siambi,Moses; Jones,Richard; Silim,Said; De Villiers,Santie. Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles... Tipo: Journal article Palavras-chave: Genetic variation; Genotyping; Microsatellites; Seed purity. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008 Registros recuperados: 2 Primeira ... 1 ... Última

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