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Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources Scientia Agricola
Mello,Marcia Ometto; Dias,Carlos Tadeu Santos; Amaral,Antônio Francisco Campos; Melo,Murilo.
Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability to utilize an alternative to sucrose as carbon source and energy for growth. Glycerol, sorbitol and galactose were the alternative carbon sources tested. Cell suspension cultures established on liquid medium containing sucrose were transferred to culture medium supplemented with sucrose or glycerol, or sorbitol, or galactose as the sole carbon source. Fresh and dry weight increasing and protein content showed marked differences among the different carbon sources used. Sucrose was the best carbon source for all the three plant species tested. Galactose and glycerol promoted slow or no growth of the three studied species....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose; Glycerol; Sorbitol; Galactose; Tissue culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300007
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Rhizobia and other legume nodule bacteria richness in brazilian Araucaria angustifolia forest Scientia Agricola
Lammel,Daniel Renato; Brancalion,Pedro Henrique Santin; Dias,Carlos Tadeu Santos; Cardoso,Elke Jurandy Bran Nogueira.
The Araucaria Forest is a sub-type of the Atlantic Forest, dominated by Araucaria angustifolia, which is considered an endangered species. The understory has a high diversity of plant species, including several legumes. Many leguminous plants nodulate with rhizobia and fix atmospheric nitrogen, contributing to forest sustainability. This work aimed at bacteria isolation and phenotypic characterization from the root nodules of legumes occurring in Araucaria Forests, at Campos do Jordão State Park, Brazil. Nodule bacteria were isolated in YMA growth media and the obtained colonies were classified according to their growth characteristics (growth rate, color, extra cellular polysaccharide production and pH change of the medium). Data were analyzed by cluster...
Tipo: Info:eu-repo/semantics/article Palavras-chave: N fixation; Ombrophilous mixed forest; Symbiotic bacteria; Phenotypical characterization.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000400011
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