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	Provedor de dados BABT (1) Scientia Agricola (1) Ciência Rural (1) Autor Dibax,Roberson (3) Quoirin,Marguerite (2) Alcantara,Giovana Bomfim de (1) Bona,Cleusa (1) Cuquel,Francine Lorena (1) Eisfeld,Cristiane de Loyola (1) Filho,João Carlos Bespalhok (1) Koehler,Henrique (1) Machado,Marília Pereira (1) Oliveira,Ricardo Augusto de (1) Quisen,Regina Caetano (1) Mais... Palavra-chave Organogenesis (2) 2 (1) 4-dichlorophenoxyacetic acid organogenesis tissue culture acclimatization Poaceae (1) Adventitious buds (1) Anatomical study (1) Callogenesis (1) Culture media (1) Forest species (1) In vitro culture (1) Tissue culture (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2013 (1) 2010 (1) 2005 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis Scientia Agricola Dibax,Roberson; Eisfeld,Cristiane de Loyola; Cuquel,Francine Lorena; Koehler,Henrique; Quoirin,Marguerite. Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L-1 NAA and 4.44 µmol L-1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Forest species; Culture media; Organogenesis. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400016 Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro BABT Dibax,Roberson; Quisen,Regina Caetano; Bona,Cleusa; Quoirin,Marguerite. The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious buds; Callogenesis; Organogenesis; Tissue culture; Anatomical study. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200009 Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars Ciência Rural Dibax,Roberson; Alcantara,Giovana Bomfim de; Machado,Marília Pereira; Filho,João Carlos Bespalhok; Oliveira,Ricardo Augusto de. The objectives of this study were to establish appropriate conditions for obtaining plant regeneration and acclimatization of the 'RB92579' and 'RB93509' sugarcane cultivars and to elucidate the shoots origin through histological analysis. For both cultivars, obtaining shoots showed better results with the culture of explants on a callus induction medium containing 2.0mg L-1 2,4-dichlorophenoxyacetic acid, followed by cultivation on a shoot induction medium containing 0.1mg L-1 kinetin and 0.2mg L-1 benzilaminopurine. The MS medium without growth regulators proved to be appropriate for elongation and rooting of shoots and the use of the composed substrate of vermiculite + MS salts was effective for acclimatization. Histological analysis revealed that the... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2; 4-dichlorophenoxyacetic acid organogenesis tissue culture acclimatization Poaceae. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100009 Registros recuperados: 3 Primeira ... 1 ... Última

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