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	Provedor de dados BJM (1) Electron. J. Biotechnol. (1) Autor Dinarvand,Mojdeh (2) B. Ariff,Arbakariya (1) Foroughi,Majid (1) Masomian,Malihe (1) Moeini,Hassan (1) Mousavi,Seyed Sadegh (1) Mustafa,Shuhaimi (1) Nahavandi,Reza (1) Rezaee,Malahat (1) Mais... Palavra-chave Aspergillus niger (1) Aspergillus niger ATCC 20611 (1) Culture optimization (1) Intra-extracellular inulinase production (1) Intra-extracellular invertase production (1) Inulinase (1) Optimization (1) Response surface method (RSM) (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2017 (1) 2012 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of extrinsic and intrinsic parameters on inulinase production by Aspergillus niger ATCC 20611 Electron. J. Biotechnol. Dinarvand,Mojdeh; B. Ariff,Arbakariya; Moeini,Hassan; Masomian,Malihe; Mousavi,Seyed Sadegh; Nahavandi,Reza; Mustafa,Shuhaimi. Background: Inulinase is a versatile enzyme from glycoside hydrolase family which targets the β-2, 1 linkage of fructopolymers. In the present study, the effect of medium composition and culture conditions on inulinase production by Aspergillus niger ATCC 20611 was investigated in shake-flasks. Results: The highest extracellular inulinase (3199 U/ ml) was obtained in the presence of 25% (w/v) sucrose, 0.5% (w/v) meat extract, 1.5% (w/v) NaNO3 and 2.5 mM (v/v) Zn2+, at initial pH of 6.5, temperature 35ºC and 6% (v/v) of spores suspension in the agitation speed of 100 rpm. Surfactants showed an inhibitory effect on enzyme production. The optimum temperature for inulinase activity was found to be 50ºC. TLC analysis showed the presence of... Tipo: Journal article Palavras-chave: Aspergillus niger ATCC 20611; Culture optimization; Inulinase. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400005 Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM) BJM Dinarvand,Mojdeh; Rezaee,Malahat; Foroughi,Majid. Abstract The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Intra-extracellular inulinase production; Intra-extracellular invertase production; Aspergillus niger; Optimization; Response surface method (RSM). Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300427 Registros recuperados: 2 Primeira ... 1 ... Última

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