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	Provedor de dados Genet. Mol. Biol. (1) PAB (1) Autor Diniz,Fábio Mendonça (2) Bentzen,Paul (1) Coimbra,Maria Raquel Moura (1) Costa,Raimundo Bezerra da (1) Iyengar,Arati (1) Lima,Paulo Sarmanho da Costa (1) Maclean,Norman (1) Maggioni,Rodrigo (1) Molina,Wagner Franco (1) Oliveira,Diana Magalhães de (1) Puchnick-Legat,Angela (1) Mais... Palavra-chave Aquaculture (1) Double-enrichment (1) Double-hybridization (1) Litopenaeus vannamei (1) Microsatellite (1) Shrimp farming (1) Stutter bands (1) Tailed microsatellite primers (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping Genet. Mol. Biol. Diniz,Fábio Mendonça; Iyengar,Arati; Lima,Paulo Sarmanho da Costa; Maclean,Norman; Bentzen,Paul. The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic library with an increased proportion of DNA fragments containing repeat motifs. Repeats were found using both types of hybridization but the double-enrichment procedure recovered sequences of which 100% contained (GATA)n and (GACA)n motifs. Microsatellite loci primers were then designed with an M13R-tail or CAG-tag to produce scorable PCR products with minimal stutter. The approach used in this study... Tipo: Info:eu-repo/semantics/article Palavras-chave: Double-enrichment; Double-hybridization; Stutter bands; Tailed microsatellite primers. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300014 Genetic variability of marine shrimp in the Brazilian industry PAB Maggioni,Rodrigo; Coimbra,Maria Raquel Moura; Costa,Raimundo Bezerra da; Diniz,Fábio Mendonça; Molina,Wagner Franco; Oliveira,Diana Magalhães de; Puchnick-Legat,Angela. The objective of this work was to estimate the genetic variability level and distribution in Brazilian broodstocks of marine shrimp (Litopenaeus vannamei). Nine of the country's largest hatcheries were evaluated using codominant and highly polymorphic microsatellite markers. The results obtained from genotyping of ten microsatellite loci are indicative of genetic variability that is compatible with that found in wild populations of L. vannamei in Mexico and Central America. A possible explanation is the highly diversified and relatively recent origin of the available broodstocks. Bayesian analysis detected a signal for five founding populations. The distribution of genetic distances partially reflects geographical location, and this information will be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Aquaculture; Microsatellite; Shrimp farming. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800026 Registros recuperados: 2 Primeira ... 1 ... Última

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