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| Auffret, Mathieu; Diouris, M; Herry, A. |
| Bivalve molluscs possess several types of hemolymph cells (hemocytes), involved in internal defense and other physiological functions. Studies in pathological and experimental conditions have shown their micro- and macrophagic ability. However, the mechanisms of intracellular digestion are incompletely known in these organisms. The lysosomal apparatus of hemocytes was therefore investigated in several species by hydrolase localization with the electron microscope. The cytoplasmic granules of granulocytes displayed a strong acid beta -glycerophosphatase activity. Previous observations had shown that these granules may contain phagocytized material. This indicates that intracellular digestion is achieved in these organelles. Acid beta -glycerophosphatase... |
| Tipo: Text |
Palavras-chave: Bivalvia; Phagocytosis; Cells; Ultrastructure; Haemolymph. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1631.pdf |
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| Auffret, Mathieu; Diouris, M; Herry, A. |
| A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical... |
| Tipo: Text |
Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf |
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