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Phage amplification assay as rapid method for Salmonella detection BJM
Siqueira,Regina Silva de; Dodd,Christine E.R.; Rees,Catherine E.D..
The application of rapid methods is crucial for the HACCP program implantation in food industry. In this context, Phage Amplification Assay is a good candidate because is based on the interactions of phage and their host bacteria. This method using phage P22 was applied with to detect Salmonella cells in chicken breast. Samples of 25 g of chicken breast were diluted and the appropriate dilutions were used in phage amplification assay for Salmonella detection. After 3-4 h of incubation, it was observed a phage titre of approximately 10(4) pfu mL-1, indicating that there were Salmonella cells which were naturally present in the meat. The presence of Salmonella cells were verified by using direct plating on XLD agar and by conventional enrichment procedure....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Rapid method; Bacteriophage; P22.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500040
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