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| Zhang,Wenjie; Li,Di; Leng,Aijing; Ai,Junjun; Du,Yang; Meng,Yihan; Gao,Yucong; Ying,Xixiang. |
| The aim of the present study was to investigate the tissue distribution and excretion of five components of Portulaca oleracea L. extract (POE) in rat following oral administration. A rapid, sensitive and specific ultra-high performance liquid chromatography (UHPLC) method with puerarin as the internal standard was used for the quantitative analysis of five components of POE, including caffeic acid (CA), p-coumaric acid (p-CA), ferulic acid (FA), quercitrin (QUER) and hesperidin (HP) in rat tissues including the liver, intestine, stomach, muscle, heart, lung, brain, kidney and spleen, urine and feces. The results show that onlyp-CA and FA were found in nearly all tissues with low cumulative ratios, and CA was higher in the intestine and stomach with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Portulaca oleracea L./pharmacokinetics; Portulaca oleracea L./extract/experimental study; Portulaca oleracea L./extract/ tissue distribution; Portulaca oleracea L./extrato/excretion; Compostos fenólicos; Ultra performance liquid chromatography/quantitative analysis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300643 |
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| Cheng,Zhongzhe; Xie,Ming; Zhang,Wenjie; Cheng,Lan; Du,Yang; Wang,Yunjiao; Ying,Xixiang; Kang,Tingguo. |
| The objective of the present study was to develop a simple and selective HPLC method for the simultaneous determination of hesperidin (HP), caffeic acid (CA), ferulic acid (FA) and p-coumaric acid (p-CA) in rat plasma after intravenous administration of Portulaca oleracea L. extract (POE). With the hyperoside as the internal standard, the sample pretreatment procedure involved simple single-step extraction with methanol of 0.2 mL plasma. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.5% glacial acetic acid (5:3:18:74, v/v/v/v). The calibration curves were linear over the range of 0.1-25 µg mL-1, 0.1-25 µg mL-1, 0.1-25 µg mL-1and 0.015-3 µg mL-1 for HP, CA, FA and p-CA, respectively. The method developed was suitable for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Portulaca oleracea L./pharmacognosy; High Performance Liquid Chromatography/rat plasma; Vegetal extract/pharmacokinetic study; Medicinal plants. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100018 |
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