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| Dettino,A.L.A.; Duarte,A.J.S.; Sato,M.N.. |
| We studied the effect of oral and portal vein administration of alloantigens on mouse skin allograft survival. Graft receptor BALB/c mice received spleen cells (30, 90, 150 or 375 x 10(6)) from donor C57BL/6 mice intragastrically on three successive days, starting seven days before the skin graft. Allograft survival was significantly increased with the feeding of 150 x 10(6) allogeneic spleen cells by one gavage (median survival of 12 vs 14 days, P <= 0.005) or when 300 x 10(6) cells were given in six gavage (12 vs 14 days, P < 0.04). A similar effect was observed when 150 x 10(6) spleen cells were injected into the portal vein (12 vs 14 days, P <= 0.03). Furthermore, prolonged allograft survival was observed with subcutaneous (12 vs 16 days, P... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse oral tolerance; Intraportal route; Skin transplantation; Interleukin-4; Alloantigen. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300022 |
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| Casseb,J.; Katzenstein,D.; Winters,M.; Brigido,L.F.M.; Duarte,A.J.S.; Hendry,R.M.. |
| The main objective of the present study was to assess the specificity and sensitivity of a modified assay using short synthetic peptides of the V3 region of HIV-1 gp120, which is the main target for neutralizing antibodies. Results from an enzyme immunoassay (EIA) employing a panel of synthetic peptides of HIV-1 subtypes and using urea washes to detect high avidity antibodies (AAV3) were compared with those obtained by the heteroduplex mobility assay and DNA sequencing. The EIA correctly typed 100% of subtype B (sensitivity = 1.0; specificity = 0.95), 100% of HIV-1 E samples (sensitivity = 1.0; specificity = 1.0), and 95% of subtype C specimens (sensitivity = 0.95; specificity = 0.94). In contrast, only 50% of subtype A (sensitivity = 0.5; specificity =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Viral diversity; Serotypes; V3 serology. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300013 |
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| Maciel,M.; Fusaro,A.E.; Oliveira,C.R.; Futata,E.A.; Duarte,A.J.S.; Sato,M.N.. |
| Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucosa; Atural IgA antibodies; Oral tolerance; Cytokines; Allergy; Dermatophagoides pteronyssinus. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006 |
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| Casseb,J.; Hong,M.A.; Gonsalez,C.; Brígido,L.F.; Duarte,A.J.S.; Michael-Hendry,R.. |
| HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HIV; Serotyping; Gp 120 V3 loop; Synthetic peptides; Sexual transmission. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000002 |
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| Komninakis,S.; Fukumori,L.; Alcalde,R.; Cortina,M.; Abdala,L.; Brito,A.; Sanabani,S.; Duarte,A.J.S.; Casseb,J.. |
| The purpose of the present study was to compare the sensitivity and specificity of V3 enzyme immunoassay (solid phase EIA and EIA inhibition) and restriction fragment length polymorphism (RFLP) with the DNA sequencing "gold standard" to identify the Brazilian HIV-1 variants of subtype B and B"-GWGR. Peripheral blood mononuclear cells were collected from 61 HIV-1-infected individuals attending a clinic in São Paulo. Proviral DNA was amplified and sequentially cleaved with the Fok I restriction enzyme. Plasma samples were submitted to a V3-loop biotinylated synthetic peptide EIA. Direct partial DNA sequencing of the env gene was performed on all samples. Based on EIA results, the sensitivity for detecting B-GPGR was 70%, compared to 64% for the Brazilian... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1 subtyping; Brazilian subtype B variant; Serotyping; DNA sequencing. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300003 |
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| Montanheiro,P.A.; Penalva de Oliveira,A.C.; Posada-Vergara,M.P.; Milagres,A.C.; Tauil,C.; Marchiori,P.E.; Duarte,A.J.S.; Casseb,J.. |
| To evaluate the human T-cell lymphotropic virus type I (HTLV-I) proviral DNA load among asymptomatic HTLV-I-infected carriers and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), real time PCR using TaqMan probes for the pol gene was performed in two million peripheral blood mononuclear cells (PBMC). The albumin gene was the internal genomic control and MT2 cells were used as positive control. The results are reported as copies/10,000 PBMC, and the detection limit was 10 copies. A total of 89 subjects (44 HAM/TSP and 45 healthy HTLV-I-infected carriers) followed up at the Institute of Infectious Diseases "Emilio Ribas" and in the Neurology Division of Hospital of Clínicas were studied. The asymptomatic HTLV-I-infected... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HTLV-I; HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-I DNA viral load. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100011 |
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