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| Romano,Eduardo; Ferreira,Adriana T.; Dusi,André N.; Proite,Karina; Buso,Jose A.; Ávila,Antonio C.; Nishijima,Marta L.; Nascimento,Adriana S.; Bravo-Almonacid,Fernando; Mentaberry,Alejandro; Monte,Damares; Campos,Magnólia A.; Melo,Paulo Eduardo; Cattony,Monica K.; Torres,Antonio C.. |
| The coat protein (CP) gene of the potato virus Y strain "o" (PVY O) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; GMO. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000200004 |
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| Dusi,André N.; Carvalho,César; Torres,Antônio Carlos; Ávila,Antonio Carlos de. |
| Two transgenic potato clones of cv. Achat, denominated 1P and 63P were challenged with two Potato virus Y strains (PVY O and PVY N), under greenhouse conditions, to be evaluated for resistance to these strains. Optical density values of the Elisa readings of samples from the transgenic plants were compared to readings from samples of the inoculated non-transformed plants. Clone 1P was extremely resistant to both PVY strains, reflected by not being systemically infected. Clone 63P, however, presented partial resistance to both PVY strains as local or systemic infection was delayed in some days. These results confirm the previously reported extreme resistance to PVY of clone 1P. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; GMO; Extreme resistance. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000300012 |
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