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	Provedor de dados PAB (3) Horticultura Brasileira (1) Autor Dusi,André Nepomuceno (4) Torres,Antonio Carlos (3) Buso,José Amauri (2) Resende,Renato de Oliveira (2) Eiras,Marcelo (1) Fajardo,Thor Vinícius (1) Ferreira,Enderson Petrônio de Brito (1) Melo Filho,Péricles de Albuquerque (1) Melo,Paulo Eduardo de (1) Nagata,Tatsuya (1) Oliveira,Cristiane Lopes de (1) Rumjanek,Norma Gouvêa (1) Xavier,Gustavo Ribeiro (1) Mais... Palavra-chave Solanum tuberosum (2) Allium sativum (1) Allium sativum L. (1) Associative bacteria (1) Coat protein (1) Coat protein gene (1) Dot-ELISA (1) Genetically modified organism (1) Heat treatment (1) Molecular marker (1) PCA (1) RT-PCR (1) Regeneration (1) Transgenic potato (1) Viral resistance (1) Virus detection (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2009 (1) 2008 (1) 2004 (1) 2000 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Shoot tip culture and thermotherapy for recovering virus-free plants of garlic Horticultura Brasileira Torres,Antonio Carlos; Fajardo,Thor Vinícius; Dusi,André Nepomuceno; Resende,Renato de Oliveira; Buso,José Amauri. Garlic shoot tip culture associated with dry heat thermotherapy (cloves exposed to 37°C for 35 days) were essential for recovering virus free plants of the cv Amarante. In this condition 70% of the explants developed in vitro and produced plants. A total of 77% of those plants was virus free when indexed by ISEM, which resulted in a final index of 54% of virus free plants from treated cloves. The percentage of regeneration decreased to 20% as the temperature increased up to 40°C. However 90% of those plants were virus free, leading to a final index of 18% virus free plants out of treated cloves. Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium sativum L.; Regeneration; Heat treatment. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362000000300010 Detection of three Allexivirus species infecting garlic in Brazil PAB Melo Filho,Péricles de Albuquerque; Nagata,Tatsuya; Dusi,André Nepomuceno; Buso,José Amauri; Torres,Antonio Carlos; Eiras,Marcelo; Resende,Renato de Oliveira. Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium sativum; Virus detection; Coat protein; Dot-ELISA; RT-PCR. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800002 Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles PAB Ferreira,Enderson Petrônio de Brito; Dusi,André Nepomuceno; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa. The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. The experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. Rhizosphere samples were collected in three sampling dates during plant development. DNA of rhizosphere microorganisms was extracted,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Associative bacteria; Molecular marker; PCA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500008 Resistance of genetically modified potatoes to Potato virus Y under field conditions PAB Dusi,André Nepomuceno; Oliveira,Cristiane Lopes de; Melo,Paulo Eduardo de; Torres,Antonio Carlos. The objective of this work was to evaluate the resistance of genetically modified clones of potato to Potato virus Y (PVY) under field conditions. Genetically modified plants were compared with nontransformed plants of the same cultivar. The plots were flanked with potato plants infected with both PVYº and PVY N strains (spread lines), in order to provide the experimental area with the source of virus, which was naturally spread by the native aphid population. The experiment was weekly monitored by visual inspections and by DAS-Elisa in the plants produced from the harvested tubers, in order to evaluate the resistance of transgenic plants throughout the plant growth cycle. By the end of the third year, no infection symptoms were observed in the 1P clone;... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Coat protein gene; Genetically modified organism; Transgenic potato; Viral resistance. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000900009 Registros recuperados: 4 Primeira ... 1 ... Última

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