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| Zahirnia,Amir Hossein; Bordbar,Ali; Ebrahimi,Sahar; Spotin,Adel; Mohammadi,Somayeh; Ghafari,Seyedeh Maryam; Ahmadvand,Setareh; Jabbari,Negar; Esmaeili Rastaghi,Ahmad Reza; Parvizi,Parviz. |
| ABSTRACT Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis. Methods All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within. Results Humans were found to be infected with L. major with high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Leishmania major; Leishmania tropica; Nuclear gene; Iran. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400278 |
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